Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Ciência e Agrotecnologia (2) OAK (1) Ciência Rural (1) Electron. J. Biotechnol. (1) Genet. Mol. Biol. (1) Autor Alderete,Liliana Marisol (1) Bodanese-Zanettini,Maria Helena (1) Bustamante,Fernanda de Oliveira (1) Callegari-Jacques,Sídia (1) Couto,Evellyn Giselly de Oliveira (1) Davide,Lisete Chamma (1) Dias,Kaio Olimpio das Graças (1) FURUOKA, Hidefumi (1) Fávero,Alessandra Pereira (1) Hagiwara,Juan Carlos (1) KOBAYASHI, Yoshiyasu (1) MATSUI, Takane (1) Pereira,Roselaine Cristina (1) Pinho,Renzo Garcia Von (1) Pinho,Édila Vilela de Resende Von (1) Salvio Escandón,Alejandro (1) Santos,Rodrigo Furtado dos (1) Santos,Vanderley Borges dos (1) Silva,Paulo Artur Konzen Xavier de Mello e (1) Simpson,Charles E. (1) Mais... Palavra-chave Colchicine (6) Apoptosis (1) Autumn crocus (Colchicum autumnale L.) (1) Brachiaria brizantha (1) Brachiaria ruziziensis (1) Cattleya (1) Chromosome number (1) Flow cytometry (1) Guinea pig (1) Induction of maternal haploids (1) Interspecific hybrids (1) Mouse (1) Nuclear DNA content (1) Orchid improvement (1) Ornamental plants (1) Peanut (1) Pollen viability (1) Polyploidization (1) Polyploidy (1) R-Navajo (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Journal article (1) Ano 2015 (2) 2012 (1) 2006 (1) 2000 (2) País Brazil (4) Chile (1) Japan (1) Idioma Inglês (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última A new variety of Bacopa monnieri obtained by in vitro polyploidization Electron. J. Biotechnol. Salvio Escandón,Alejandro; Hagiwara,Juan Carlos; Alderete,Liliana Marisol. The commercial value of Bacopa monnieri, a widespread herbaceous plant in Argentina, can be substantially improved increasing its flower size by chromosome doubling with colchicine. MS supplemented with 0.25 mg/L of 6-benzylaminopurine proved to be an appropriate medium for the in vitro multiplication of nodal segments of B. monnieri. Polyploidization was achieved submerging nodal segments during 24 or 48 hrs in colchicine solution 0.001 and 0.01% P/V, in 1% DMSO. Segments submerged in water and in DMSO 1% aqueous solution were used as controls. DNA contents from recovered plants was measured and characterized and their phenotype described. Two tetraploid plants originated from independent events were detected. These plants showed significant differences... Tipo: Journal article Palavras-chave: Colchicine; Flow cytometry; Ornamental plants; Scrophulariaceae; Tetraploid. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300002 Comparison of Enterotoxicity between Autumn Crocus (Colchicum autumnale L.) and Colchicine in the Guinea Pig and Mouse : Enterotoxicity in the Guinea Pig Differs from That in the Mouse OAK YAMADA, Manabu; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; MATSUI, Takane; 古林, 与志安; 古岡, 秀文. Autumn crocus poisoning of cattle is characterized by severe diarrhea caused by alkaloid colchicine. Previously, we examined pathologically this poisoning in cattle and reported that enterotoxic lesions were closely associated with apoptosis. To examine enterotoxicity of autumn crocus more precisely, a reproductive study was performed using guinea pigs and mice, and pathological findings associated with autumn crocus poisoning were compared with those of colchicine. Each group of guinea pigs given the bulb of autumn crocus or colchicine exhibited severe diarrhea. Histopathological findings in intoxicated guinea pigs were entirely consistent with those in the autumn crocus-poisoned cattle. In contrast, each group of mice administered with the bulb or... Palavras-chave: Apoptosis; Autumn crocus (Colchicum autumnale L.); Colchicine; Guinea pig; Mouse. Ano: 2000 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4170 Histogram score contributes for reliability of DNA content estimatives in Brachiaria spp Ciência e Agrotecnologia Timbó,Ana Luiza de Oliveira; Pereira,Roselaine Cristina; Santos,Vanderley Borges dos; Souza Sobrinho,Fausto; Davide,Lisete Chamma. Flow cytometry allows to estimate the DNA content of a large number of plants quickly. However, inadequate protocols can compromise the reliability of these estimates leading to variations in the values of DNA content the same species. The objective of this study was to propose an efficient protocol to estimate the DNA content of Brachiaria spp. genotypes with different ploidy levels using flow cytometry. We evaluated four genotypes (B. ruziziensis diploid and artificially tetraploidized; a tetraploid B. brizantha and a natural triploid hybrid), three buffer solutions (MgSO4, Galbraith and Tris-HCl) and three species as internal reference standards (Raphanus sativus, Solanum lycopersicum e Pisum sativum). The variables measured were: histogram score (1-5),... Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria ruziziensis; Brachiaria brizantha; Colchicine; Nuclear DNA content. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000600001 In vivo HAPLOID INDUCTION AND EFFICIENCY OF TWO CHROMOSOME DUPLICATION PROTOCOLS IN TROPICAL MAIZE Ciência e Agrotecnologia Couto,Evellyn Giselly de Oliveira; Pinho,Édila Vilela de Resende Von; Pinho,Renzo Garcia Von; Veiga,Adriano Delly; Bustamante,Fernanda de Oliveira; Dias,Kaio Olimpio das Graças. ABSTRACTArtificial chromosome duplication is one of the most important process in the attainment of doubled haploids in maize. This study aimed to evaluate the induction ability of the inducer line KEMS in a tropical climate and test the efficiency of the R1-Navajo marker by flow cytometry to evaluate two chromosome duplication protocols and analyze the development of the doubled haploids in the field. To accomplish this goal, four genotypes (F1 and F2 generations) were crossed with the haploid inducer line KEMS. The seeds obtained were selected using the R1-Navajo marker and subject to two chromosome duplication protocols. Duplication was confirmed using flow cytometry. The percentages of self-fertilized plants after duplication as well as the quantities... Tipo: Info:eu-repo/semantics/article Palavras-chave: R-Navajo; Colchicine; Induction of maternal haploids; Zea mays. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000500435 Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques Ciência Rural Silva,Paulo Artur Konzen Xavier de Mello e; Callegari-Jacques,Sídia; Bodanese-Zanettini,Maria Helena. The effect of colchicine on the protocorm-like bodies of Cattleya intermedia L. (Orchidaceae) cultured in vitro was studied. Different concentrations of the drug (0.00, 0.05, 0.10 and 0.20%) as well as two times of culture (4 or 8 days) were tested to determine the best treatment for the induction of tetraploid plants. Of the 3 colchicine levels applied, 0.05 and 0.10% of drug treatments appeared to be effective on the production of mixoploids and tetraploids and could be further used in breeding programs. The suitability of stomatal area and stomata density as criteria for the distinction between diploids and tetraploids was also tested. The results suggested that tetraploid plants could be identified with a fair amount of certainty when the screening was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattleya; Colchicine; Chromosome number; Orchid improvement; Polyploidy; Stomata analysis. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000100017 Successful crosses between fungal-resistant wild species of Arachis (section Arachis) and Arachis hypogaea Genet. Mol. Biol. Fávero,Alessandra Pereira; Santos,Rodrigo Furtado dos; Simpson,Charles E.; Valls,José Francisco Montenegro; Vello,Natal Antonio. Peanut (Arachis hypogaea) is the fifth most produced oil crop worldwide. Besides lack of water, fungal diseases are the most limiting factors for the crop. Several species of Arachis are resistant to certain pests and diseases. This study aimed to successfully cross the A-genome with B-K-A genome wild species previously selected for fungal disease resistance, but that are still untested. We also aimed to polyplodize the amphihaploid chromosomes; cross the synthetic amphidiploids and A. hypogaea to introgress disease resistance genes into the cultivated peanut; and analyze pollen viability and morphological descriptors for all progenies and their parents. We selected 12 A-genome accessions as male parents and three B-genome species, one K-genome species,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Peanut; Interspecific hybrids; Polyploidization; Colchicine; Pollen viability. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300353 Registros recuperados: 6 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco